IDI – Ricerca Scientifica

Il Centro di Allergologia Molecolare (CAM) è attivamente impegnato nella ricerca clinica, traslazionale, e di base nel campo delle malattie allergiche e immuno-mediate.
Il focus più importante è sulle malattie allergiche mediate da anticorpi IgE quali l'asma, la rinite, l'orticaria, l'anafilassi, l'eczema, e la congiuntivite.
Il CAM è stato il primo centro clinico al mondo nel 2006 ad implementare la diagnostica con allergeni molecolari basata su tecnologia su microarray (ISAC test, VBC-Genomics, Vienna, Austria) come diagnostica di routine. Questa implementazione ha previsto ricerca e sviluppo nel campo delle strutture allergeniche (numerosi nuovi allergeni sono stati identificati, caratterizzati e prodotti), e del loro impatto clinico-epidemiologico. In aggiunta al test ISAC attualmente utilizzato nella routine diagnostica (ISAC 112, Phadia Austria, Vienna, Austria), sono stati messi a punto alcuni nuovi microarray con molecole allergeniche sperimentali. Questa procedura permette lo studio di nuove specificità antigeniche, che in tempi successivi verrano introdotte nella routine.
Per tutte queste finalità il CAM utilizza una infrastruttura di "information technology" basata su una cartella elettronica (InterAll) interconnessa con l'unica piattaforma esistente attualmente in Internet dedicata agli allergeni molecolari e non (www.allergome.org). La cartella elettronica permette la gestione dei dati demografici, clinici e diagnostici del CAM con un collegamento real time tra il centro clinico (ambulatori e DH) e il laboratorio, dove si svolgono le attività di routine e di ricerca. I dati disponibili nel database InterAll-CAM vengono analizzati e gestiti a fini di ricerca mediante procedure bioinformatiche sviluppate ad hoc dai ricercatori del CAM.

Presso il CAM è attivo anche un servizio di citofluorimetria. Sempre a carattere di ricerca i test in citofluorimetria vengono utilizzati per lo studio dell'attivazione dei basofili, per la definizione delle sottopopolazioni linfocitarie in diverse condizioni patologiche, e per gli studi di proliferazione cellulare. Sempre con metodica citofluorimetrica i ricercatori del CAM hanno messo a punto un nuovo sistema per la determinazione delle IgE in microtecnologia. Questo nuovo sistema chiamato ABA permetterà una ulteriore personalizzazione del profilo diagnostico di ogni singolo paziente.

Il personale del CAM è diviso in tre settori: Clinico, Biologico, Bioinformatico

Direttore del CAM:
Dr. Adriano Mari, adriano.mari@idi.it

 

Assistente
Dr.ssa Silvia Monti s.monti@idi.it

Settore Clinico:
Dr.ssa Claudia Alessandri (Allergologia Pediatrica) c.alessandri@idi.it
Dr.ssa Maria Livia Bernardi ml.bernardi@idi.it
Dr.ssa Rosetta Ferrara r.ferrara@idi.it
Dr.ssa Danila Zennaro d.zennaro@idi.it

Settore Biologico:
Dr.ssa Paola Palazzo p.palazzo@hotmail.it
Dr.ssa Debora Pomponi dpomponi@libero.it
Dr.ssa Lisa Tuppo l.tuppo@ibp.cnr.it
Dr.ssa Ivana Giangrieco° i.giangrieco@ibp.cnr.it
Dr.ssa Marina Liso° marinaliso@libero.it

Dr.ssa Roberta Crescenzo° r.crescenzo@ibp.cnr.it
Sig.na Chiara Rafaiani c.rafaiani@hotmail.it

Settore Bioinformatico:
Dr.ssa Sara Zuzzi° sara.zuzzi@gmail.com
Dr. Mario Santoro° santoro.ma@gmail.com


Il CAM intrattiene numerose collaborazioni con Centri di Ricerca Nazionali e Internazionali:

• Allergy and Asthma Center, Johns Hopkins University, Baltimore, USA - Prof. Robert Hamilton
• Christian Doppler Laboratory for Allergy Diagnosis and Therapy Department of Molecular Biology, University of Salzburg, Austria - Prof. Fatima Ferreira
• Christian Doppler Laboratory for Allergy Research, Division of Immunopathology, Department of Pathophysiology, Center of Physiology and Pathophysiology, Medical University of Vienna, Austria - Prof. Rudolf Valenta
• Department of Medicine, Tulane University, New Orleans, USA Prof. Samuel B. Lehrer
• Department of Pathophysiology, Center of Physiology and Pathophysiology, Medical University of Vienna, Austria - Prof. Barbara Bohle
• Department of Pathophysiology, Center of Physiology and Pathophysiology, Medical University of Vienna, University of Vienna, Austria - Prof. Erika Jensen-Jarolim
• Department of Pediatrics, Mount Sinai University, New York, USA - Prof. Xiu-Min Lin
• Department of Pediatrics, University of São Paulo, Ribeirão Preto, Brazil - Prof. Karla Arruda
• Department of Pharmacology, School of Pharmacy, Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem, Israel - Prof. Francesca Levi-Schaffer
• Food Allergy Research & Resource Program - University of Nebraska – Lincoln, NE, USA - Prof. Richard E. Goodman
• Institute for Protein Biochemistry, Consiglio Nazionale delle Ricerche, Naples, Italy - Prof. Maria Antonietta Ciardiello
• Institute of Immunology and Allergology, Inselspital, Bern, Switzerland - Prof. Beda Stadler
• Institute of Pathophysiology, University of Vienna, Austria - Prof. Heimo Breiteneder
• Laboratory of Immunogenetics and Allergology, CRP-Santé, Luxembourg - Prof. Annette Kuehn
• Research Center Borstel - Division of Molecular and Clinical Allergology, Leibniz – Center for Medicine and Biosciences, Borstel, Germany - Dr. Arnd Petersen
• University of Paranà, Cutiriba, Brazil - Prof. Nelson Rosario
• USDA-ARS-SRRC, New Orleans, LA, USA - Prof. Soheila J. Maleki

Le più recenti pubblicazioni scientifiche del CAM:

- Twaroch TE, Focke-Tejkl M, Civaj V, Weber M, Balic N, Mari A, Ferrara R, Quirce S, Spitzauer S, Valenta R, Swoboda I. Carrier-bound, nonallergenic Ole e 1 peptides for vaccination against olive pollen allergy. J Allergy Clin Immunol (2011);accepted.
- Scala E, Abeni D, Palazzo P, Liso M, Pomponi D, Lombardo G, Picchio C, Russo G, Mari A. Specific IgE toward allergenic molecules is a new prognostic marker in patients with Sézary Syndrome. Int Arch Allergy Immunol (2011); accepted.
- Alessandri C, Scala E, Zennaro D, Ferrara R, Bernardi ML, Mari A. Molecule-based diagnosis in allergy. Riv Immunol Allergol Pediatr (2010);5:11-20.
- Egger M, Alessandri C, Wallner M, Briza P, Zennaro D, Mari A, Ferreira F, Gadermaier G. Studying the allergenic potential of different chicken breeds: Do ancient hens lay better eggs? PLOS One(2011); accepted
- Schulten V, Nagla B, Scala E, Bernardi ML, Mari A, Ciardiello MA, Lauer I, Scheurer S, Jürets A, Briza P, Ferreira F, Bohle B.
Pru p 3, the non-specific lipid transfer protein from peach, dominates the cellular and humoral response to its homolog in hazelnut Cor a 8.
Allergy (2011); epub ahead of print
- Radauer C, Adhami F, Fürtler I, Wagner S, Allwardt D, Scala E, Ebner C, Hafner C, Hemmer W, Mari A, Breiteneder H
Sensitization patterns and cross-reactivity of hevein and hevein-like domains from class I chitinases among latex-allergic patients.
Mol Immunol (2011); 48:600-9.
- D'Avino R, Bernardi ML, Wallner M, Palazzo P, Camardella L, Tuppo L, Alessandri C, Breiteneder H, Ferreira F, Ciardiello MA, Mari A.
Kiwifruit Act d 11 is the first member of the Ripening-related Protein family identified as an allergen.
Allergy (2011); epub ahead of print
- Magni C, Ballabio C, Restani P, Fuggetta D, Alessandri C, Mari A, Bernardini R, Dello Iacono I, Arlorio M, Duranti M.
Molecular insight into IgE-mediated reactions to sesame (Sesamum indicum, L.) seed proteins.
Ann Allergy Asthma Immunol (2010); 105:458-64.
- Gadermaier G, Egger M, Girbl T, Erler A, Harrer A, Vejvar E, Liso M, Richter K, Zuidmeer L, Mari A, Ferreira F.
Molecular characterization of Api g 2, a novel allergenic member of the lipid-transfer protein 1 family from celery stalks.
Mol Nutr Food Res (2011); 55:568-77.
- Mari A, Ciardiello MA, Tamburrini M, Rasi C, Palazzo P.
Proteomic analysis in the identification of allergenic molecules: current procedures, evolving role of microarray technology and future strategies.
Expert Rev Proteomics (2010); 7:723-34.
- Bublin M, Dennstedt S, Buchegger M, Ciardiello MA, Bernardi ML, Harwanegg C, Hafner C, Ballmer-Weber B, Knulst A, Hoffmann-Sommergruber K, Mari A, Breiteneder H.
The performance of a component-based allergen microarray for the diagnosis of kiwifruit allergy.
Clin Exp Allergy (2011);41:129-36.
- Bernardi ML, Picone D, Tuppo L, Giangrieco I, Petrella G, Palazzo P, Ferrara R, Tamburini M, Mari A, Ciardiello MA.
Physico-chemical features of the environment affect the protein conformation and the immunoglobulin E reactivity of kiwellin (Act d 5).
Clin Exp Allergy (2010); 40:1819-26.
- Hochwallner H, Schulmeister U, Swoboda I, Balic N, Geller B, Nystrand M, Härlin A, Thalhamer J, Scheiblhofer S, Niggemann B, Quirce S, Ebner C, Mari A, Pauli G, Herz U, Van Tol EAF, Valenta R, Spitzauer S.
Microarray and allergenic activity assessment of milk allergens.
Clin Exp Allergy (2010); 40:1809-18.
- Scala E, Abeni D, Pomponi D, Narducci MG, Lombardo GA, Mari A, Frontani M, Picchio MC, Pilla MA, Caprini E, Russo G.
Predictive role of 9-O-acetylated ganglioside D3 (CD60) and α4β1 (CD49d) expression in the survival of patients with Sezary Syndrome.
Haematologica (2010); 95:1905-12.
- Mari A, Alessandri C, Bernardi ML, Ferrara R, Scala E, Zennaro D. Microarrayed Allergen Molecules for the Diagnosis of Allergic Diseases.
Curr Allergy Asthma Rep (2010); 10:357-64.
- Egger M, Hauser M, Mari A, Ferreira F, Gadermaier G. The Role of Lipid Transfer Proteins in Allergic Diseases.
Curr Allergy Asthma Rep (2010); 10:326-35.
- Pfiffner P, Truffer R, Matsson P, Rasi C, Mari A, Stadler BM. Allergen cross reactions: A problem greater than ever thought?
Allergy (2010); 65:1536-44.
- Scala E, Alessandri C, Bernardi ML, Ferrara R, Palazzo P, Pomponi D, Quaratino D, Rasi C, Zaffiro A, Zennaro D, Mari A.  Cross-sectional survey on IgE reactivity in 23,077 subjects using an allergenic molecule-based microarray detection system.
Clin Exp Allergy (2010);40:911-21.
- Hochwallner H, Schulmeister U, Swoboda I, Focke-Tejkl M, Civaj V, Balic N, Nystrand M, Härlin A, Thalhamer J, Scheiblhofer S, Keller W, Pavkov T, Zafred D, Niggemann B, Quirce S, Mari A, Pauli G, Ebner C, Papadopoulos NG, Herz U, Van Tol EAF, Valenta R, Spitzauer S
Visualization of clustered IgE epitopes on α-lactalbumin.
J Allergy Clin Immunol (2010);125:1279-85.
- Ciardiello MA, Palazzo P, Bernardi ML, Carratore V, Giangrieco I, Longo V, Melis M, Tamburrini M, Zennaro D, Mari A, Colombo P.
Biochemical, immunological and clinical characterization of a cross-reactive non-specific lipid transfer protein 1 from mulberry.
Allergy (2010);65:597-605.
- Pokoj S, Lauer I, Fötisch K, Himly M, Mari A, Enrique E, San Miguel-Moncin M, Lidholm J, Vieths S, Scheurer S.
Pichia pastoris is superior to E. coli for the production of recombinant allergenic non-specific Lipid Transfer Proteins.
Protein Expr Purif (2010); 69:68-75.
- Schulmeister U, Hochwallner H, Swoboda I, Focke-Tejkl M, Geller B, Nystrand M, Härlin A, Thalhamer J, Scheiblhofer S, Keller W, Niggemann B, Quirce S, Ebner C, Mari A, Pauli G, Herz U, Valenta R, Spitzauer S
Cloning, expression, and mapping of allergenic determinants of alphaS1-casein, a major cow's milk allergen.
J Immunol (2009); 182:7019-29.
- Alessandri C, Zennaro D, Zaffiro A, Mari A.  Molecular allergology approach to allergic diseases in the paediatric age.
Ital J Ped (2009);35(1):29 epub.
- Shahali Y, Pourpak Z, Moin M, Mari A, Majd A
Immunoglobulin E reactivity to Arizona cypress pollen extracts: evidence for a 35-kDa allergen.
Allergy (2009);64:1687-8.
- Mari A, Rasi C, Palazzo P, Scala E.  Allergen Databases: Current status and Perspectives.
Curr Allergy Asthma Rep (2009); 9:376-83.
- Krause S, Reese G, Randow S, Zennaro D, Quaratino D, Palazzo P, Ciardiello MA, Petersen A, Becker W-M, Mari A
The Lipid Transfer Protein (Ara h 9) as a New Peanut Allergen relevant for a Mediterranean Allergic Population.
J Allergy Clin Immunol (2009); 124:771-8.e5.
- Schulmeister U, Hochwallner H, Swoboda I, Focke-Tejkl M, Geller B, Nystrand M, Härlin A, Thalhamer J, Scheiblhofer S, Keller W, Niggemann B, Quirce S, Ebner C, Mari A, Pauli G, Herz U, Valenta R, Spitzauer S.
Cloning, Expression and Mapping of Allergenic Determinants of AlphaS1-casein, a
Major Cow's Milk Allergen.   J Immunol (2009);182:7019-7029.
- Shahali Y, Pourpak Z, Moin M, Mari A, Majd A.  Instability of the structure and allergenic protein content in Arizona cypress pollen.
Allergy (2009); 64:1773-9.
- Gadermaier G, Harrer A, Girbl T, Palazzo P, Himly M, Briza P, Mari A, Ferreira F.
Isoform identification and characterization of Art v 3, the lipid-transfer protein of mugwort pollen.
Mol Immunol (2009);46:1919-24.
- Schulten V, Radakovics A, Hartz C, Mari A, Vazquez-Cortes S, Fernandez-Rivas M, Lauer I, Jahn-Schmid B, Eiwegger T, Scheurer S, Bohle B
Characterization of the allergic T cell response to Pru p 3, the non-specific lipid transfer protein in peach.
J Allergy Clin Immunol (2009);124:100-7.
- Constantin C, Quirce S, Poorafshar M, Touraev A, Nystrand M, Niggemann B, Mari A, Akerström H, Heberle-Bors E, Valenta R
Micro-arrayed wheat seed and grass pollen allergens for component resolved diagnosis.
Allergy (2009);64:1030-7.
- Ciardiello MA , Giangrieco I, Tuppo L, Tamburini M, Buccheri M, Palazzo P, Bernardi ML, Ferrara R, Mari A.
Influence of the natural ripening stage, cold storage and ethylene treatment on the protein and IgE-binding profiles of green and gold kiwi fruit extracts.
J Agric Food Chem (2009);57:1565-71.
- Pöll V, Denk U, Shen H-D, Panzani RC, Dissertori O, Lackner P, Hemmer W, Mari A, Crameri R, Lottspeich F, Rid R, Richter K, Breitenbach M, Simon-Nobbe B.
The vacuolar serine protease, a cross-reactive allergen from Cladosporium herbarum.
Mol Immunol (2009);46:1360-73.
- Wallner M, Erler A, Hauser M, Klinglmayr E, Gadermaier G, Vogel L, Mari A, Bohle B, Briza P, Ferreira F.
Immunologic characterization of isoforms of Car b 1 and Que a 1, the major hornbeam and oak pollen allergens.
Allergy (2009);64:452-60.